LOCALIZATION OF ACID MUCOPOLYSACCHARIDES WITH BASIC FUCHSIN

Variability of haematoxylin - basic fuchsin - picric acid (HBFP) staining of infarcted myocardium due to tissue storage.

Early myocardial infarction is usually detected (3) The effect of storage and drying on the timgrossly by utilising the nitro-blue tetrazolium (NBT) ing of the differentiation step was also technique.1 The normal areas stain dark blue and studied. infarcted areas which lack the dehydrogenase enzyme remain unstained. In 1971, Lie et aI2 described a non-enzymatic histochemical method, the haemato...

Acid mucopolysaccharides in normal serum.

Mucopolysaccharide which contains uronic acid has recently been described in normal plasma (1, 2). A method for the quantitation of plasma acid mucopolysaccharide was described by Badin, Schubert and Vouras (1), based on the observation that chondroitin sulfate added to plasma is found in euglobulin precipitates (3). These authors reported an increased amount of acid mucopolysaccharide in the p...

Urinary Excretion of Acid Mucopolysaccharides by Patients with Rheumatoid Arthritis'

Several methods have been described for the determination of urinary acid mucopolysaccharides (1-4). It has been found that in normal individuals the daily urinary excretion varies with age and sex (4, 5). A few attempts have been made to correlate urinary excretion of acid mucopolysaccharides with different disease states. Craddock and Kerby (6) have reported that patients with impairment of r...

Quantitative Colorimetric Assay of Acid Mucopolysaccharides

In 1946 Hale (1) described a histochemical method for the detection of acid polysaccharides in animal tissues, based on the ability of these compounds to bind ferric ions, which were subsequently detected as Prussian blue. It has been possible to adapt this histochemical method to the quantitative determination of acid mucopolysaccharides on a macro or micro scale. The major modification of the...

The Localization of Acid Mucopolysaccharides in the Golgi Complex of Intestinal Goblet Cells